UV-C dose required to kill microorganisms

Below is a list of microorganisms and the UV-C dose at 254 nm (displayed in J/m2) required to achieve a 90% (1 log reduction).

UV dose (J/m2) = UV irradiance (W/m2) x exposure time (seconds)

If you know the UV irradiance value then you can use our online UV dose calculator to determine the UV dose applied, which varies with time.

In order to calculate further log reductions, you could – as a guide – multiply the D90 value to further reduce contamination as follows:

  • D90 multiplied by 2 could provide you with a 99% (2 log) reduction
  • D90 multiplied by 3 could provide you with a 99.9% (3 log) reduction
  • D90 multiplied by 4 could provide you with a 99.99% (4 log) reduction
  • D90 multiplied by 5 could provide you with a 99.999% (5 log) reduction
  • D90 multiplied by 6 could provide you with a 99.9999% (6 log) reduction

However, it is recommended that trials and evaluation are conducted to determine the required UV dose levels required to achieve different log reductions of your target microorganisms.



Coronavirus (SARS-CoV-2)

Since the start of the SARS CoV-2 pandemic, Dr. Anthony Griffiths, Associate Professor of Microbiology at Boston University School of Medicine and his team have been working on developing tools to support scientific advancement in this field.

During their research, they have treated inoculated material with different doses of UV-C radiation and assessed the inactivation capacity under various conditions. The team applied a dose of 50 J/m2, resulting in a reduction of the SARS-CoV-2 virus of 99%. Based on the data, it was determined that a dose of 220 J/m2 will result in a reduction of 99.9999%.

“Our test results show that above a specific dose of UV-C radiation, viruses were completely inactivated; in a matter of seconds we could no longer detect any virus,” said Dr. Anthony Griffiths.

Bacteria

Microbe Type D90 Media Source
Acinetobacter baumannii Veg 18 J/m2 Surface Rastogi 2007
B. atrophaeus (B. globigii) Sp 144 J/m2 Air EPA 2006
Bacillius anthracis spores Sp 45 J/m2 Surface Sharp 1939
Bacillius anthracis spores Sp 743 J/m2 Surface Knudson 1986
Bacillus cereus spores Sp 267 J/m2 Surface Weinberger 1984
Bacillus cereus spores Sp 210 J/m2 Surface Weisova 1966
Bacillus cereus spores Sp 116 J/m2 Surface Germaine 1973
Bacillus cereus spores Sp 408 J/m2 Surface Benoit 1990
Bacillus megatherium Sp 273 J/m2 Surface Hercik 1937
Bacillus megatherium Veg 113 J/m2 Surface Hercik 1937
Bacillus subtilis Veg 14 J/m2 Air Nakamura 1987
Bacillus subtilis spores Sp 94 J/m2 Surface Rentschler 1941
Bacillus subtilis spores Sp 68 J/m2 Surface Munakata 1975
Bacillus subtilis spores Sp 113 J/m2 Surface Munakata 1975
Bacillus subtilis spores Sp 89 J/m2 Air Peccia 2001a
Bacillus subtilis spores Sp 149 J/m2 Air Ke 2009
Bacillus subtilis spores Sp 85 J/m2 Air Peccia 2001a
Burkholderia cepacia Veg 22 J/m2 Air Fletcher 2004
Corynebacterium diphtheriae Veg 33 J/m2 Surface Sharp 1939
Escherichia coli Veg 25 J/m2 Surface Sharp 1939
Escherichia coli Veg 19 J/m2 Surface Rentschler 1942
Escherichia coli Veg 12 J/m2 Surface Rentschler 1942
Escherichia coli Veg 25 J/m2 Surface Rentschler 1941
Escherichia coli Veg 20 J/m2 Surface Quek 2008
Escherichia coli Veg 51 J/m2 Surface Luckiesh 1949
Escherichia coli Veg 34 J/m2 Surface Kim 2002
Escherichia coli Veg 55 J/m2 Surface Hollaender 1955
Escherichia coli Veg 8 J/m2 Surface Collins 1971
Escherichia coli Veg 3 J/m2 Air Webb 1970
Escherichia coli Veg 11 J/m2 Air Webb 1970
Escherichia coli Veg 11 J/m2 Air Rentschler 1942
Escherichia coli Veg 13 J/m2 Air Rentschler 1942
Escherichia coli Veg 15 J/m2 Air Luckiesh 1949
Escherichia coli Veg 2 J/m2 Air Koller 1939
Escherichia coli Veg 11 J/m2 Air Koller 1939
Francisella tularensis Veg 256 J/m2 Air Beebe 1959
Francisella tularensis Veg 288 J/m2 Air Beebe 1959
Haemophilus influenzae Veg 38 J/m2 Surface Mongold 1992
Halobacterium sp. NRC-1 Veg 25 J/m2 Surface Crowley 2006
Legionella dumoffi Veg 24 J/m2 Surface Knudson 1985
Legionella bozemanii Veg 15 J/m2 Surface Knudson 1985
Legionella gormani Veg 26 J/m2 Surface Knudson 1985
Legionella jordanis Veg 11 J/m2 Surface Knudson 1985
Legionella longbeach Veg 11 J/m2 Surface Knudson 1985
Legionella micdadei Veg 15 J/m2 Surface Knudson 1985
Legionella oakridgensis Veg 22 J/m2 Surface Knudson 1985
Legionella pneumophila Veg 5 J/m2 Surface Knudson 1985
Legionella wadsworthii Veg 4 J/m2 Surface Knudson 1985
Listeria monocytogenes Veg 156 J/m2 Surface Kim 2002
Listeria monocytogenes Veg 10 J/m2 Surface Collins 1971
Micrococcus candidus Veg 61 J/m2 Surface Hollaender 19855
Micrococcus piltonesis Veg 81 J/m2 Surface Rentschler 1941
Micrococcus sphaeroides Veg 100 J/m2 Surface Rentschler 1941
Mycobacterium bovis BCG Veg 22 J/m2 Surface Collins 1971
Mycobacterium bovis BCG Veg 10 J/m2 Air Riley 1976
Mycobacterium bovis BCG Veg 12 J/m2 Air Peccia 2002
Mycobacterium bovis BCG Veg 19 J/m2 Air Ko 2000
Mycobacterium bovis BCG Veg 33 J/m2 Air Ko 2000
Mycobacterium parafortuitum Veg 13 J/m2 Air Peccia 2001
Mycobacterium parafortuitum Veg 46 J/m2 Air Peccia 2001
Mycobacterium parafortuitum Veg 19 J/m2 Air Xu 2003
Mycobacterium phlei Veg 63 J/m2 Air Riley 1976
Mycobacterium phlei Veg 23 J/m2 Air Kethley 1973
Mycobacterium phlei Veg 16 J/m2 Air Gillis 1974
Mycobacterium smegmatis Veg 12 J/m2 Air Gillis 1974
Mycobacterium tuberculosis Veg 11 J/m2 Surface Collins 1971
Mycobacterium tuberculosis Veg 5 J/m2 Air Riley 1976
Mycoplasma arthritidis Veg 7 J/m2 Surface Furness 1977
Mycoplasma fermentans Veg 9 J/m2 Surface Furness 1977
Mycoplasma hominis Veg 7 J/m2 Surface Furness 1977
Mycoplasma Orale type 1 Veg 11 J/m2 Surface Furness 1977
Mycoplasma Orale type 2 Veg 6 J/m2 Surface Furness 1977
Mycoplasma pheumoniae Veg 8 J/m2 Surface Furness 1977
Mycoplasma salivarium Veg 11 J/m2 Surface Furness 1977
Neisseria catarrhalis Veg 44 J/m2 Surface Rentschler 1941
Nocardia asteroides Veg 280 J/m2 Surface Chick 1963
Phytomonas tumefaciens Veg 44 J/m2 Surface Rentschler 1941
Proteus vulgaris Veg 30 J/m2 Surface Rentschler 1941
Pseudomonas aeruginosa Veg 55 J/m2 Surface Hollaender 1955
Pseudomonas aeruginosa Veg 22 J/m2 Surface Elasri 1999
Pseudomonas aeruginosa Veg 10 J/m2 Surface Collins 1971
Pseudomonas aeruginosa Veg 4 J/m2 Air Sharp 1940
Pseudomonas fluorescens Veg 35 J/m2 Surface Rentschler 1941
Pseudomonas fluorescens Veg 3 J/m2 Air van Osdell 2002
Salmonella enteritidis Veg 10 J/m2 Surface Collins 1971
Salmonella typhi Veg 21 J/m2 Surface Sharp 1939
Sarcina lutea Veg 197 J/m2 Surface Rentschler 1941
Serratia indica Veg 209 J/m2 Air Harstad 1954
Serratia marcescens Veg 22 J/m2 Surface Sharp 1939
Serratia marcescens Veg 22 J/m2 Surface Rentschler 1941
Serratia marcescens Veg 8 J/m2 Surface Hollaender 1955
Serratia marcescens Veg 10 J/m2 Surface Collins 1971
Serratia marcescens Veg 2 J/m2 Air Fletcher 2003
Serratia marcescens Veg 24 J/m2 Air Fletcher 2003
Serratia marcescens Veg 8 J/m2 Air UVDI 2001
Serratia marcescens Veg 4 J/m2 Air Ko 2000
Serratia marcescens Veg 115 J/m2 Air Ko 2000
Serratia marcescens Veg 5 J/m2 Air Sharp 1940
Serratia marcescens Veg 20 J/m2 Air Nakamura 1987
Serratia marcescens Veg 33 J/m2 Air Peccia 2001
Serratia marcescens Veg 3 J/m2 Air Lai 2004
Serratia marcescens Veg 3 J/m2 Air van Osdell 2002
Serratia marcescens Veg 5 J/m2 Air Peccia 2001
Serratia marcescens Veg 1 J/m2 Air Lai 2004
Shigella paradysenteriae Veg 17 J/m2 Surface Sharp 1939
Spirillum rubrum Veg 44 J/m2 Surface Rentschler 1941
Staphylococcus albus Veg 18 J/m2 Surface Sharp 1939
Staphylococcus albus Veg 33 J/m2 Surface Rentschler 1941
Staphylococcus albus (1) Veg 23 J/m2 Air Rentschler 1942
Staphylococcus albus (2) Veg 52 J/m2 Air Rentschler 1942
Staphylococcus aureus Veg 30 J/m2 Surface Sturm 1932
Staphylococcus aureus Veg 50 J/m2 Surface Hollaender 1955
Staphylococcus aureus Veg 66 J/m2 Surface Gates 1934
Staphylococcus aureus Veg 26 J/m2 Surface Sharp 1939
Staphylococcus aureus Veg 37 J/m2 Surface Luckiesh 1949
Staphylococcus aureus Veg 19 J/m2 Surface Gates 1929
Staphylococcus aureus Veg 20 J/m2 Air Nakamura 1987
Staphylococcus aureus Veg 7 J/m2 Air Sharp 1940
Staphylococcus aureus Veg 2 J/m2 Air Luckiesh 1949
Staphylococcus aureus Veg 2 J/m2 Air Luckiesh 1946
Staphylococcus epidermis Veg 14 J/m2 Air van Osdell 2002
Staphylococcus epidermis Veg 29 J/m2 Air van Osdell 2002
Staphylococcus epidermis Veg 20 J/m2 Air Nakamura 1987
Staphylococcus epidermis Veg 22 J/m2 Air Furuhashi 1989
Streptococcus agalactiae Veg 5 J/m2 Air Luckiesh 1949
Streptococcus haemolyticus Veg 22 J/m2 Surface Sharp 1939
Streptococcus pneumoniae Veg 468 J/m2 Surface Gritz 1990
Streptococcus pyogenes Veg 4 J/m2 Surface Lidwell 1950
Streptococcus pyogenes Veg 1 J/m2 Air Luckiesh 1949
Stroptococcus viridans Veg 20 J/m2 Surface Sharp 1939

Viruses

Microbe Type D90 Media Source
Adenovirus dsDNA  34 J/m2 Air Walker 2007
Adenovirus dsDNA 59 J/m2 Air Walker 2007
Adenovirus dsDNA 42 J/m2 Air Jensen 1964
Adenovirus type 2 dsDNA 400 J/m2 Surface Day 1974
Adenovirus type 40 dsDNA 300 J/m2 Surface Meng 1996
Adenovirus type 41 dsDNA 240 J/m2 Surface Meng 1996
Bacteriophage MS2 dsRNA 26 J/m2 Air Walker 2007
Bacteriophage MS2 dsRNA 61 J/m2 Air Walker 2007
Bacteriophage MS2 dsRNA 3 J/m2 Air Tseng 2005
Bacteriophage MS2 dsRNA 4 J/m2 Air Tseng 2005
Coliphage X-174 dsDNA 3 J/m2 Air Tseng 2005
Coliphage X-174 dsDNA 4 J/m2 Air Tseng 2005
Coliphage PRD1 dsDNA 87 J/m2 Surface Meng 1996
Coliphage T7 dsDNA 7 J/m2 Air Tseng 2005
Coliphage T7 dsDNA 10 J/m2 Air Tseng 2005
Coronavirus ssRNA 3 J/m2 Air Walker 2007
Coxsackievirus ssRNA 21 J/m2 Air Jensen 1964
Human Cytomegalovirus dsDNA 658 J/m2 Surface Albrecht 1974
Human Cytomegalovirus dsDNA 50 J/m2 Surface Jensen 1964
Influenza A virus ssRNA 19 J/m2 Air Jensen 1964
Newcastle Disease Virus ssRNA 16 J/m2 Surface Rubin 1959
phage phi 6 dsRNA 5 J/m2 Air Tseng 2005
phage phi 6 dsRNA 7 J/m2 Air Tseng 2005
Poliovirus dsRNA 44 J/m2 Surface Ma 1994
Poloiovirus type 1 dsRNA 41 J/m2 Surface Meng 1996
Rauscher Murine Leukemia v. ssRNA 959 J/m2 Surface Stull 1976
Rous Sarcoma virus (RSV) ssRNA 200 J/m2 Surface Rubin 1959
Sindbis virus ssRNA 62 J/m2 Air Jensen 1964
S. aureus phage ssDNA 82 J/m2 Surface Gates 1934
S. aureus phage ssDNA 77 J/m2 Surface Sturm 1932
Vaccina virus ssDNA 1 J/m2 Air McDevitt 2007
Vaccina virus ssDNA 15 J/m2 Air Jensen 1964
Trustpilot